- Cdh1 Mutants
- cdh1∆
- sic1∆ cdh1∆
- sic1∆ cdh1∆ GALL-CDC20
- sic1∆ cdc6∆2-49 cdh1∆
- sic1∆ cdc6∆2-49 cdh1∆ GALL-CDC20
- cdh1∆ cdc6∆2-49
- swi5∆ cdh1∆
- swi5∆ cdh1∆ GAL-SIC1
- cln1∆ cln2∆ cdh1∆
- GAL-CLN2 cln1∆ cln2∆ cdh1∆
- cln1∆ cln2∆ cln3∆ cdh1∆
- GAL-SIC1 cln1∆ cln2∆ cdh1∆
- GAL-SIC1 GAL-CLN2 cln1∆ cln2∆ cdh1∆
- CLB1 clb2∆ cdh1∆
- GAL-CLB2 cdh1∆
- GAL-CLB5 cdh1∆
- cdc14-ts cdh1∆
- cdc15∆ net1-ts cdh1∆
- APC-A cdh1∆
- APC-A cdh1∆ in galactose
- APC-A cdh1∆ GAL-SIC1
- APC-A cdh1∆ GAL-CDC6
- APC-A cdh1∆ multi-copy SIC1
- APC-A cdh1∆ multicopy CDC6
- APC-A cdh1∆ multicopy CDC20
- Cdh1 constitutively active
Cdh1 constitutively active
debug: ,
test user =
test db =
Simulation:
Change of parameters: kicdh"=0, kscdh=0.03, MDT=150.
Length of G1 phase: 36.7 min.
Arrest: G2 arrest
Experiments:
Zachariae, W., Schwab, M., Nasmyth, K. and Seufert, W. (1998). Control of cyclin ubiquitination by CDK-regulated binding of Hct1 to the anaphase promoting complex. Science 282:1721-1724.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Fig. 4, cells containing GALLp-HA3-Hct1-m11 failed to accumulate mitotic cyclin Clb2 and Clb3, and failed to form mitotic spindle or to undergo cytokinesis.
(Hct1-m11, where all eleven potential Cdk phosphorylation sites on Hct1 are removed, is constitutively active. GALL promoter is a weak galactose-inducible promoter.)
(Hct1-m11, where all eleven potential Cdk phosphorylation sites on Hct1 are removed, is constitutively active. GALL promoter is a weak galactose-inducible promoter.)
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